Primer Details for TRINITY_DN67523_c3_g6_i1 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAGAAGTAAGTGTTTGAAGTTTCGA | TGTGCAAAATTCGCTCTCAAA | Start: 7 |
| Tm | 59.668 | 57.293 | End: 119 |
| Size | 27 | 21 | 113 |
| Primer 2 details |
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| Sequence | TCAAGAAGTAAGTGTTTGAAGTTTCGA | TTGTGCAAAATTCGCTCTCAA | Start: 7 |
| Tm | 59.668 | 57.293 | End: 120 |
| Size | 27 | 21 | 114 |
| Primer 3 details |
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| Sequence | TCAAGAAGTAAGTGTTTGAAGTTTCGA | TTTGTGCAAAATTCGCTCTCA | Start: 7 |
| Tm | 59.668 | 57.293 | End: 121 |
| Size | 27 | 21 | 115 |
| Primer 4 details |
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| Sequence | TGAAGTTTCGAAATTACATAGCTCCT | TTTTGTGCAAAATTCGCTCTCA | Start: 23 |
| Tm | 58.896 | 57.961 | End: 122 |
| Size | 26 | 22 | 100 |
| Primer 5 details |
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| Sequence | CAAGAAGTAAGTGTTTGAAGTTTCGA | TGTGCAAAATTCGCTCTCAAA | Start: 8 |
| Tm | 58.539 | 57.293 | End: 119 |
| Size | 26 | 21 | 112 |
