ssr_primer
Primer Details for TRINITY_DN67517_c2_g1_i3 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCATAGGTGCGATCGGTG | GATGGTGGAGGGATTGGCAA | Start: 168 |
| Tm | 59.896 | 60.033 | End: 285 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | TTGCCAATCCCTCCACCATC | TAGCGTGCCATGGACTTCTG | Start: 266 |
| Tm | 60.033 | 60.108 | End: 469 |
| Size | 20 | 20 | 204 |
| Primer 3 details |
|---|
| Sequence | AGTGCACTTCCGTCACAGTT | AGGAGGGCCCAAAAGTAAGC | Start: 601 |
| Tm | 59.822 | 59.96 | End: 733 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | AGTGCACTTCCGTCACAGTT | TTTCAGAAGGCGAGTGCACT | Start: 601 |
| Tm | 59.822 | 59.893 | End: 763 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | TTGCCAATCCCTCCACCATC | ACATAGCGTGCCATGGACTT | Start: 266 |
| Tm | 60.033 | 59.747 | End: 472 |
| Size | 20 | 20 | 207 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCATAGGTGCGATCGGTG | GATGGTGGAGGGATTGGCAA | Start: 168 |
| Tm | 59.896 | 60.033 | End: 285 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | TTGCCAATCCCTCCACCATC | TAGCGTGCCATGGACTTCTG | Start: 266 |
| Tm | 60.033 | 60.108 | End: 469 |
| Size | 20 | 20 | 204 |
| Primer 3 details |
|---|
| Sequence | AGTGCACTTCCGTCACAGTT | AGGAGGGCCCAAAAGTAAGC | Start: 601 |
| Tm | 59.822 | 59.96 | End: 733 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
|---|
| Sequence | AGTGCACTTCCGTCACAGTT | TTTCAGAAGGCGAGTGCACT | Start: 601 |
| Tm | 59.822 | 59.893 | End: 763 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | TTGCCAATCCCTCCACCATC | ACATAGCGTGCCATGGACTT | Start: 266 |
| Tm | 60.033 | 59.747 | End: 472 |
| Size | 20 | 20 | 207 |
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