ssr_primer
Primer Details for TRINITY_DN67473_c1_g1_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCCGTACCCTGTAGAAGG | CGGCAATGGCATGTTCCAAA | Start: 4750 |
| Tm | 60.033 | 60.038 | End: 4905 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | TAATGTGACACCCAAGCGCT | AACTACTCGAAGGGCACGTG | Start: 3457 |
| Tm | 59.964 | 60.039 | End: 3604 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | TAATGTGACACCCAAGCGCT | CCTGGGGTGGAGAGAGTACA | Start: 3457 |
| Tm | 59.964 | 59.959 | End: 3709 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
|---|
| Sequence | CACGTGCCCTTCGAGTAGTT | CCTGGGGTGGAGAGAGTACA | Start: 3585 |
| Tm | 60.039 | 59.959 | End: 3709 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | GTAATGTGACACCCAAGCGC | AACTACTCGAAGGGCACGTG | Start: 3456 |
| Tm | 59.832 | 60.039 | End: 3604 |
| Size | 20 | 20 | 149 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCCGTACCCTGTAGAAGG | CGGCAATGGCATGTTCCAAA | Start: 4750 |
| Tm | 60.033 | 60.038 | End: 4905 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | TAATGTGACACCCAAGCGCT | AACTACTCGAAGGGCACGTG | Start: 3457 |
| Tm | 59.964 | 60.039 | End: 3604 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | TAATGTGACACCCAAGCGCT | CCTGGGGTGGAGAGAGTACA | Start: 3457 |
| Tm | 59.964 | 59.959 | End: 3709 |
| Size | 20 | 20 | 253 |
| Primer 4 details |
|---|
| Sequence | CACGTGCCCTTCGAGTAGTT | CCTGGGGTGGAGAGAGTACA | Start: 3585 |
| Tm | 60.039 | 59.959 | End: 3709 |
| Size | 20 | 20 | 125 |
| Primer 5 details |
|---|
| Sequence | GTAATGTGACACCCAAGCGC | AACTACTCGAAGGGCACGTG | Start: 3456 |
| Tm | 59.832 | 60.039 | End: 3604 |
| Size | 20 | 20 | 149 |
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