ssr_primer
Primer Details for TRINITY_DN67448_c3_g1_i2 |
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| (A)14tatt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCCACAGCTTTACGCAGA | GCACGGAGTTGTTGGAAACC | Start: 177 |
| Tm | 60.037 | 59.97 | End: 347 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
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| Sequence | ACACGACGTGACGGAAAAGA | GCACGGAGTTGTTGGAAACC | Start: 133 |
| Tm | 59.899 | 59.97 | End: 347 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
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| Sequence | CCTCCACAGCTTTACGCAGA | TGCTTAAGCTTGCACGGAGT | Start: 177 |
| Tm | 60.037 | 60.25 | End: 358 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
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| Sequence | CCTCCACAGCTTTACGCAGA | TTGGGTCGATAGGTGGCAAG | Start: 177 |
| Tm | 60.037 | 59.748 | End: 387 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
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| Sequence | CCTCCACAGCTTTACGCAGA | CTTGGGTCGATAGGTGGCAA | Start: 177 |
| Tm | 60.037 | 59.748 | End: 388 |
| Size | 20 | 20 | 212 |
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