ssr_primer
Primer Details for TRINITY_DN67422_c4_g1_i6 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGACGGGGCATCAGATA | ACGATCGAGCAACAGACAGG | Start: 3297 |
| Tm | 60.106 | 60.109 | End: 3466 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | TCCAGACGGGGCATCAGATA | CGATCGAGCAACAGACAGGA | Start: 3297 |
| Tm | 60.106 | 59.829 | End: 3465 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | CACCTTGGACCCTCAGCTTC | CTGGATGATTTGCTTCGCCG | Start: 2218 |
| Tm | 60.322 | 59.971 | End: 2425 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | TGGGGAGACATTGCAATGGG | CTGGATGATTTGCTTCGCCG | Start: 2312 |
| Tm | 60.325 | 59.971 | End: 2425 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | GGGGAGACATTGCAATGGGT | CTGGATGATTTGCTTCGCCG | Start: 2313 |
| Tm | 60.325 | 59.971 | End: 2425 |
| Size | 20 | 20 | 113 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGACGGGGCATCAGATA | ACGATCGAGCAACAGACAGG | Start: 3297 |
| Tm | 60.106 | 60.109 | End: 3466 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | TCCAGACGGGGCATCAGATA | CGATCGAGCAACAGACAGGA | Start: 3297 |
| Tm | 60.106 | 59.829 | End: 3465 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | CACCTTGGACCCTCAGCTTC | CTGGATGATTTGCTTCGCCG | Start: 2218 |
| Tm | 60.322 | 59.971 | End: 2425 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | TGGGGAGACATTGCAATGGG | CTGGATGATTTGCTTCGCCG | Start: 2312 |
| Tm | 60.325 | 59.971 | End: 2425 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | GGGGAGACATTGCAATGGGT | CTGGATGATTTGCTTCGCCG | Start: 2313 |
| Tm | 60.325 | 59.971 | End: 2425 |
| Size | 20 | 20 | 113 |
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