ssr_primer
Primer Details for TRINITY_DN67401_c7_g1_i4 |
|---|
| (G)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCGAGCCAAAATTAAGCG | TTCAATGGAGGACACCCAGC | Start: 249 |
| Tm | 59.904 | 59.962 | End: 469 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | CAGGCGTTGTCCTTGCAAAA | TTCAATGGAGGACACCCAGC | Start: 313 |
| Tm | 59.898 | 59.962 | End: 469 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | GGAGTCCCGTGCATCTTCAA | GATTGCGAGTTGACTGCAGC | Start: 827 |
| Tm | 60.036 | 60.179 | End: 934 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | TTGTCCTTGCAAAAACGGGC | TTCAATGGAGGACACCCAGC | Start: 319 |
| Tm | 60.179 | 59.962 | End: 469 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | GGAGTCCCGTGCATCTTCAA | GTGTCCCCCAGACTCTGAGA | Start: 827 |
| Tm | 60.036 | 60.252 | End: 1038 |
| Size | 20 | 20 | 212 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCGAGCCAAAATTAAGCG | TTCAATGGAGGACACCCAGC | Start: 249 |
| Tm | 59.904 | 59.962 | End: 469 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | CAGGCGTTGTCCTTGCAAAA | TTCAATGGAGGACACCCAGC | Start: 313 |
| Tm | 59.898 | 59.962 | End: 469 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | GGAGTCCCGTGCATCTTCAA | GATTGCGAGTTGACTGCAGC | Start: 827 |
| Tm | 60.036 | 60.179 | End: 934 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | TTGTCCTTGCAAAAACGGGC | TTCAATGGAGGACACCCAGC | Start: 319 |
| Tm | 60.179 | 59.962 | End: 469 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | GGAGTCCCGTGCATCTTCAA | GTGTCCCCCAGACTCTGAGA | Start: 827 |
| Tm | 60.036 | 60.252 | End: 1038 |
| Size | 20 | 20 | 212 |
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