ssr_primer
Primer Details for TRINITY_DN67367_c2_g2_i2 |
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| (C)17tatatgtgacttttgcgttttttctaaactcggtctattgaagtaagtgcccttttgtaacaaacaaac(A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCCATAGACAGCCCCTTTT | GGGGGTGAGTCGAGAGTAGT | Start: 248 |
| Tm | 60.033 | 60.034 | End: 491 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
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| Sequence | ACCGACGCGTGTAATTGACT | CCTGGCTTGCCTGGATAACA | Start: 683 |
| Tm | 60.039 | 60.034 | End: 805 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
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| Sequence | ACTACTCTCGACTCACCCCC | AGTCAATTACACGCGTCGGT | Start: 472 |
| Tm | 60.034 | 60.039 | End: 702 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
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| Sequence | ACGCCTCTTCACATCGACAG | CCTGGCTTGCCTGGATAACA | Start: 659 |
| Tm | 60.109 | 60.034 | End: 805 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
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| Sequence | ACTACTCTCGACTCACCCCC | CTGTCGATGTGAAGAGGCGT | Start: 472 |
| Tm | 60.034 | 60.109 | End: 678 |
| Size | 20 | 20 | 207 |
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