ssr_primer
Primer Details for TRINITY_DN67365_c1_g2_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGTCAACTTTGCCACATT | TTCGTCACACATGGGAAGGG | Start: 932 |
| Tm | 59.968 | 59.964 | End: 1163 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CCCTTCCCATGTGTGACGAA | CGGACAGGAGGAACGTTCAA | Start: 1144 |
| Tm | 59.964 | 59.967 | End: 1320 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
|---|
| Sequence | AACTTGCCGTCAACTTTGCC | TTCGTCACACATGGGAAGGG | Start: 927 |
| Tm | 59.898 | 59.964 | End: 1163 |
| Size | 20 | 20 | 237 |
| Primer 4 details |
|---|
| Sequence | GCCGTCAACTTTGCCACATT | CGTCACACATGGGAAGGGAT | Start: 932 |
| Tm | 59.968 | 59.747 | End: 1161 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | ATCCCTTCCCATGTGTGACG | CGGACAGGAGGAACGTTCAA | Start: 1142 |
| Tm | 59.747 | 59.967 | End: 1320 |
| Size | 20 | 20 | 179 |
(T)10ctccgatagtttgaagaaagattggggggattacgcaccctataaaaaat(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGTCAACTTTGCCACATT | TTCGTCACACATGGGAAGGG | Start: 932 |
| Tm | 59.968 | 59.964 | End: 1163 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CCCTTCCCATGTGTGACGAA | CGGACAGGAGGAACGTTCAA | Start: 1144 |
| Tm | 59.964 | 59.967 | End: 1320 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
|---|
| Sequence | AACTTGCCGTCAACTTTGCC | TTCGTCACACATGGGAAGGG | Start: 927 |
| Tm | 59.898 | 59.964 | End: 1163 |
| Size | 20 | 20 | 237 |
| Primer 4 details |
|---|
| Sequence | GCCGTCAACTTTGCCACATT | CGTCACACATGGGAAGGGAT | Start: 932 |
| Tm | 59.968 | 59.747 | End: 1161 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | ATCCCTTCCCATGTGTGACG | CGGACAGGAGGAACGTTCAA | Start: 1142 |
| Tm | 59.747 | 59.967 | End: 1320 |
| Size | 20 | 20 | 179 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGTCAACTTTGCCACATT | TTCGTCACACATGGGAAGGG | Start: 932 |
| Tm | 59.968 | 59.964 | End: 1163 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CCCTTCCCATGTGTGACGAA | CGGACAGGAGGAACGTTCAA | Start: 1144 |
| Tm | 59.964 | 59.967 | End: 1320 |
| Size | 20 | 20 | 177 |
| Primer 3 details |
|---|
| Sequence | AACTTGCCGTCAACTTTGCC | TTCGTCACACATGGGAAGGG | Start: 927 |
| Tm | 59.898 | 59.964 | End: 1163 |
| Size | 20 | 20 | 237 |
| Primer 4 details |
|---|
| Sequence | GCCGTCAACTTTGCCACATT | CGTCACACATGGGAAGGGAT | Start: 932 |
| Tm | 59.968 | 59.747 | End: 1161 |
| Size | 20 | 20 | 230 |
| Primer 5 details |
|---|
| Sequence | ATCCCTTCCCATGTGTGACG | CGGACAGGAGGAACGTTCAA | Start: 1142 |
| Tm | 59.747 | 59.967 | End: 1320 |
| Size | 20 | 20 | 179 |
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