ssr_primer
Primer Details for TRINITY_DN67342_c4_g6_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCAATGGGGTTTACAGCG | AGGGGAGAGTTGGGAGAAAA | Start: 52 |
| Tm | 60.109 | 57.866 | End: 222 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
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| Sequence | GCAATGGGGTTTACAGCGAC | AGGGGAGAGTTGGGAGAAAA | Start: 54 |
| Tm | 59.83 | 57.866 | End: 222 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
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| Sequence | TGCAATGGGGTTTACAGCGA | AGGGGAGAGTTGGGAGAAAA | Start: 53 |
| Tm | 60.251 | 57.866 | End: 222 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
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| Sequence | TCATCTCCCTTCTGACCGGA | AGGGGAGAGTTGGGAGAAAA | Start: 116 |
| Tm | 59.665 | 57.866 | End: 222 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
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| Sequence | GTGCAATGGGGTTTACAGCG | AGGGGAGAGTTGGGAGAAAAT | Start: 52 |
| Tm | 60.109 | 58.351 | End: 222 |
| Size | 20 | 21 | 171 |
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