ssr_primer
Primer Details for TRINITY_DN67341_c6_g1_i2 |
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| (A)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTTGTTTGCAAACTGAGGCA | TTTGCTACCCCCTCAATGGC | Start: 519 |
| Tm | 60.135 | 60.324 | End: 685 |
| Size | 21 | 20 | 167 |
| Primer 2 details |
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| Sequence | CTCAATGTGGGTTGGCTCCT | CCCTCTCATTGTGTTGGAGGA | Start: 90 |
| Tm | 59.961 | 59.37 | End: 322 |
| Size | 20 | 21 | 233 |
| Primer 3 details |
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| Sequence | CTCAATGTGGGTTGGCTCCT | TCCCTCTCATTGTGTTGGAGG | Start: 90 |
| Tm | 59.961 | 59.37 | End: 323 |
| Size | 20 | 21 | 234 |
| Primer 4 details |
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| Sequence | TGCACCTATCTGCATACGGA | TTTGCTACCCCCTCAATGGC | Start: 457 |
| Tm | 58.591 | 60.324 | End: 685 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
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| Sequence | GGTTGTTTGCAAACTGAGGCA | CCCCTCAATGGCGGGAAATT | Start: 519 |
| Tm | 60.135 | 60.687 | End: 677 |
| Size | 21 | 20 | 159 |
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