ssr_primer
Primer Details for TRINITY_DN67334_c1_g1_i1 |
|---|
| (A)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATGGGTTTCATCCGTCC | GCGATGATACTCTGCTCCCC | Start: 947 |
| Tm | 60.034 | 60.038 | End: 1137 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | ACCATGGGTTTCATCCGTCC | GGCGATGATACTCTGCTCCC | Start: 947 |
| Tm | 60.034 | 60.038 | End: 1138 |
| Size | 20 | 20 | 192 |
| Primer 3 details |
|---|
| Sequence | CCATGGGTTTCATCCGTCCA | GCGATGATACTCTGCTCCCC | Start: 948 |
| Tm | 60.035 | 60.038 | End: 1137 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | CCATGGGTTTCATCCGTCCA | GGCGATGATACTCTGCTCCC | Start: 948 |
| Tm | 60.035 | 60.038 | End: 1138 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | ACCATGGGTTTCATCCGTCC | AGCAAGGAGTGGACGTAACG | Start: 947 |
| Tm | 60.034 | 60.039 | End: 1162 |
| Size | 20 | 20 | 216 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCATGGGTTTCATCCGTCC | GCGATGATACTCTGCTCCCC | Start: 947 |
| Tm | 60.034 | 60.038 | End: 1137 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | ACCATGGGTTTCATCCGTCC | GGCGATGATACTCTGCTCCC | Start: 947 |
| Tm | 60.034 | 60.038 | End: 1138 |
| Size | 20 | 20 | 192 |
| Primer 3 details |
|---|
| Sequence | CCATGGGTTTCATCCGTCCA | GCGATGATACTCTGCTCCCC | Start: 948 |
| Tm | 60.035 | 60.038 | End: 1137 |
| Size | 20 | 20 | 190 |
| Primer 4 details |
|---|
| Sequence | CCATGGGTTTCATCCGTCCA | GGCGATGATACTCTGCTCCC | Start: 948 |
| Tm | 60.035 | 60.038 | End: 1138 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | ACCATGGGTTTCATCCGTCC | AGCAAGGAGTGGACGTAACG | Start: 947 |
| Tm | 60.034 | 60.039 | End: 1162 |
| Size | 20 | 20 | 216 |
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