ssr_primer
Primer Details for TRINITY_DN67304_c7_g1_i11 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCGGAACTAGATGCATCG | GCGACAGAAGCATGTTCGTG | Start: 844 |
| Tm | 59.967 | 60.179 | End: 957 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | AGCCGGAACTAGATGCATCG | GCATGTTCGTGAATGGTGCA | Start: 844 |
| Tm | 59.967 | 59.759 | End: 948 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | GCAATAGCCTTGAACCGCTC | TCCGGGTTCTTGAGAGCAAC | Start: 81 |
| Tm | 59.621 | 59.965 | End: 295 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | GCAATAGCCTTGAACCGCTC | CCGGGTTCTTGAGAGCAACT | Start: 81 |
| Tm | 59.621 | 59.965 | End: 294 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | GCCGGAACTAGATGCATCGT | GCGACAGAAGCATGTTCGTG | Start: 845 |
| Tm | 60.249 | 60.179 | End: 957 |
| Size | 20 | 20 | 113 |
(T)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCCGGAACTAGATGCATCG | GCGACAGAAGCATGTTCGTG | Start: 844 |
| Tm | 59.967 | 60.179 | End: 957 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | AGCCGGAACTAGATGCATCG | GCATGTTCGTGAATGGTGCA | Start: 844 |
| Tm | 59.967 | 59.759 | End: 948 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | GCAATAGCCTTGAACCGCTC | TCCGGGTTCTTGAGAGCAAC | Start: 81 |
| Tm | 59.621 | 59.965 | End: 295 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | GCAATAGCCTTGAACCGCTC | CCGGGTTCTTGAGAGCAACT | Start: 81 |
| Tm | 59.621 | 59.965 | End: 294 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | GCCGGAACTAGATGCATCGT | GCGACAGAAGCATGTTCGTG | Start: 845 |
| Tm | 60.249 | 60.179 | End: 957 |
| Size | 20 | 20 | 113 |
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