ssr_primer
Primer Details for TRINITY_DN67263_c4_g1_i8 |
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| (A)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGGGCCAATTTCCATGAT | TATAGAGCTGATGCGCAGGC | Start: 66 |
| Tm | 60.032 | 60.038 | End: 290 |
| Size | 20 | 20 | 225 |
| Primer 2 details |
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| Sequence | CTATCGCTGCAGGGCTACTC | GCCATTCCGGTTAGCTTTGC | Start: 1246 |
| Tm | 60.039 | 60.179 | End: 1361 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
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| Sequence | ACCGGGCCAATTTCCATGAT | CCACCGCTCGACTTGAAGTA | Start: 66 |
| Tm | 60.032 | 59.757 | End: 336 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
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| Sequence | GCCTGCGCATCAGCTCTATA | ATCAAAGTGGACGCTTCCGA | Start: 271 |
| Tm | 60.038 | 59.68 | End: 378 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
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| Sequence | CTATCGCTGCAGGGCTACTC | TCCGGTTAGCTTTGCTTCCC | Start: 1246 |
| Tm | 60.039 | 60.322 | End: 1356 |
| Size | 20 | 20 | 111 |
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