ssr_primer
Primer Details for TRINITY_DN67259_c2_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCATTTGCGCGTGAACGT | ATTAGCGTAGAAGCGGACCG | Start: 650 |
| Tm | 59.972 | 59.97 | End: 759 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTTCATTTGCGCGTGAACGT | GGTCATTGCATTTGCGCTCA | Start: 650 |
| Tm | 59.972 | 60.109 | End: 786 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | TTTCATTTGCGCGTGAACGT | TGGTCATTGCATTTGCGCTC | Start: 650 |
| Tm | 59.972 | 60.109 | End: 787 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | CGGTCCGCTTCTACGCTAAT | TCCGGACTTGTAGACACCCT | Start: 740 |
| Tm | 59.97 | 59.886 | End: 983 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | GGTCAGGGACGTAACGGTAC | ACGTTCACGCGCAAATGAAA | Start: 511 |
| Tm | 59.829 | 59.972 | End: 669 |
| Size | 20 | 20 | 159 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCATTTGCGCGTGAACGT | ATTAGCGTAGAAGCGGACCG | Start: 650 |
| Tm | 59.972 | 59.97 | End: 759 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTTCATTTGCGCGTGAACGT | GGTCATTGCATTTGCGCTCA | Start: 650 |
| Tm | 59.972 | 60.109 | End: 786 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | TTTCATTTGCGCGTGAACGT | TGGTCATTGCATTTGCGCTC | Start: 650 |
| Tm | 59.972 | 60.109 | End: 787 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | CGGTCCGCTTCTACGCTAAT | TCCGGACTTGTAGACACCCT | Start: 740 |
| Tm | 59.97 | 59.886 | End: 983 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | GGTCAGGGACGTAACGGTAC | ACGTTCACGCGCAAATGAAA | Start: 511 |
| Tm | 59.829 | 59.972 | End: 669 |
| Size | 20 | 20 | 159 |
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