ssr_primer
Primer Details for TRINITY_DN67212_c0_g2_i4 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCAGCACGGTTCATGGAAA | ATGCAGAACAAGAGGACGCA | Start: 123 |
| Tm | 59.89 | 59.965 | End: 281 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
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| Sequence | TGGAAATCCAGCACGGTTCA | ATGCAGAACAAGAGGACGCA | Start: 117 |
| Tm | 59.89 | 59.965 | End: 281 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
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| Sequence | AGACGGAAAGCGATCGGTTT | TTAGATGCCATGCCATCGCT | Start: 1986 |
| Tm | 60.038 | 59.891 | End: 2231 |
| Size | 20 | 20 | 246 |
| Primer 4 details |
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| Sequence | AGACGGAAAGCGATCGGTTT | ACCTACCGTGGTTGCCAAAT | Start: 1986 |
| Tm | 60.038 | 59.888 | End: 2157 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
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| Sequence | AGACGGAAAGCGATCGGTTT | CAGTCTAACGTAGCCGCTGT | Start: 1986 |
| Tm | 60.038 | 59.829 | End: 2184 |
| Size | 20 | 20 | 199 |
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