ssr_primer
Primer Details for TRINITY_DN67181_c2_g1_i5 |
|---|
| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCTCTATGTGCGCCAAGT | CCAACTGTATGGCTGGTCGT | Start: 353 |
| Tm | 60.036 | 60.036 | End: 606 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
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| Sequence | TGCGTTGGGTAGCTACCTTG | ACTTGGCGCACATAGAGCTT | Start: 258 |
| Tm | 60.037 | 60.036 | End: 372 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
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| Sequence | ACGACCAGCCATACAGTTGG | GCTTCGAAACTCTCACGGGA | Start: 587 |
| Tm | 60.036 | 60.039 | End: 721 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
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| Sequence | ATGAGGATGCCTACACCCCT | TAAAGTATCGGGTCGCCTGC | Start: 278 |
| Tm | 60.03 | 59.896 | End: 411 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | CCTGAGACAGAAGCCAGTCG | GCAGCGAAATAACTCTGCCG | Start: 900 |
| Tm | 60.109 | 59.973 | End: 1055 |
| Size | 20 | 20 | 156 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCTCTATGTGCGCCAAGT | CCAACTGTATGGCTGGTCGT | Start: 353 |
| Tm | 60.036 | 60.036 | End: 606 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | TGCGTTGGGTAGCTACCTTG | ACTTGGCGCACATAGAGCTT | Start: 258 |
| Tm | 60.037 | 60.036 | End: 372 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | ACGACCAGCCATACAGTTGG | GCTTCGAAACTCTCACGGGA | Start: 587 |
| Tm | 60.036 | 60.039 | End: 721 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | ATGAGGATGCCTACACCCCT | TAAAGTATCGGGTCGCCTGC | Start: 278 |
| Tm | 60.03 | 59.896 | End: 411 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | CCTGAGACAGAAGCCAGTCG | GCAGCGAAATAACTCTGCCG | Start: 900 |
| Tm | 60.109 | 59.973 | End: 1055 |
| Size | 20 | 20 | 156 |
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