ssr_primer
Primer Details for TRINITY_DN67170_c2_g1_i1 |
|---|
| (T)10a(T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTCCGAACAATGTTAGCG | ACTTCGAGTCGGTTTCGCTT | Start: 1273 |
| Tm | 59.975 | 59.968 | End: 1490 |
| Size | 20 | 20 | 218 |
| Primer 2 details |
|---|
| Sequence | CGCTCCGAACAATGTTAGCG | GTTTCGCTTACGCCGTTGTT | Start: 1273 |
| Tm | 59.975 | 60.042 | End: 1479 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | AAGCGAAACCGACTCGAAGT | CCACCGTTTATCCCCGTGAA | Start: 1471 |
| Tm | 59.968 | 60.036 | End: 1701 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | AGTTTTCTCGGCCATGCAGA | ACTTCGAGTCGGTTTCGCTT | Start: 1304 |
| Tm | 59.963 | 59.968 | End: 1490 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | AAGCGAAACCGACTCGAAGT | ATCCCCGTGAAAACCCCTTC | Start: 1471 |
| Tm | 59.968 | 59.961 | End: 1692 |
| Size | 20 | 20 | 222 |
(T)16(G)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTCCGAACAATGTTAGCG | ACTTCGAGTCGGTTTCGCTT | Start: 1273 |
| Tm | 59.975 | 59.968 | End: 1490 |
| Size | 20 | 20 | 218 |
| Primer 2 details |
|---|
| Sequence | CGCTCCGAACAATGTTAGCG | GTTTCGCTTACGCCGTTGTT | Start: 1273 |
| Tm | 59.975 | 60.042 | End: 1479 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
|---|
| Sequence | AAGCGAAACCGACTCGAAGT | CCACCGTTTATCCCCGTGAA | Start: 1471 |
| Tm | 59.968 | 60.036 | End: 1701 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | AGTTTTCTCGGCCATGCAGA | ACTTCGAGTCGGTTTCGCTT | Start: 1304 |
| Tm | 59.963 | 59.968 | End: 1490 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | AAGCGAAACCGACTCGAAGT | ATCCCCGTGAAAACCCCTTC | Start: 1471 |
| Tm | 59.968 | 59.961 | End: 1692 |
| Size | 20 | 20 | 222 |
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