ssr_primer
Primer Details for TRINITY_DN67159_c6_g2_i2 |
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| (A)10caaaaaaccccccaaaaaatccagaacaccttcgacacttctgatattttcaggactttgtcaagggggactttgt(C)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCCCACAGTTTAGTCGCT | AAGGATGGAAGGGGCATGTG | Start: 380 |
| Tm | 60.037 | 60.033 | End: 487 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
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| Sequence | AAGGAGACCGAACTTTGGGC | TGTGTTGCTCTCGTCCAGAC | Start: 288 |
| Tm | 60.251 | 59.968 | End: 471 |
| Size | 20 | 20 | 184 |
| Primer 3 details |
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| Sequence | GTCTGGACGAGAGCAACACA | AGGAGCCCTAGACGATACCG | Start: 452 |
| Tm | 59.968 | 60.251 | End: 551 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | AAGGAGACCGAACTTTGGGC | AAGGATGGAAGGGGCATGTG | Start: 288 |
| Tm | 60.251 | 60.033 | End: 487 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
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| Sequence | AAGGAGACCGAACTTTGGGC | AGCGACTAAACTGTGGGCTC | Start: 288 |
| Tm | 60.251 | 60.037 | End: 399 |
| Size | 20 | 20 | 112 |
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