Primer Details for TRINITY_DN67053_c4_g1_i1 |
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| (A)38: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAATGGGCCTAGTCCTCCT | GTCCAGTTTTAATCAAGGAGTTCTT | Start: 71 |
| Tm | 59.657 | 57.488 | End: 225 |
| Size | 20 | 25 | 155 |
| Primer 2 details |
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| Sequence | TTGAATGGGCCTAGTCCTCC | GTCCAGTTTTAATCAAGGAGTTCTT | Start: 70 |
| Tm | 58.787 | 57.488 | End: 225 |
| Size | 20 | 25 | 156 |
| Primer 3 details |
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| Sequence | TTGAATGGGCCTAGTCCTCCT | GTCCAGTTTTAATCAAGGAGTTCTT | Start: 70 |
| Tm | 60.274 | 57.488 | End: 225 |
| Size | 21 | 25 | 156 |
| Primer 4 details |
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| Sequence | TGGGCCTAGTCCTCCTAGAA | GTCCAGTTTTAATCAAGGAGTTCTT | Start: 75 |
| Tm | 58.684 | 57.488 | End: 225 |
| Size | 20 | 25 | 151 |
| Primer 5 details |
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| Sequence | ATGGGCCTAGTCCTCCTAGA | GTCCAGTTTTAATCAAGGAGTTCTT | Start: 74 |
| Tm | 58.453 | 57.488 | End: 225 |
| Size | 20 | 25 | 152 |
