ssr_primer
Primer Details for TRINITY_DN67047_c2_g1_i5 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATCCGAGTCTGCTCTCTGC | ATGAGCAGCTCTGGCAAAGT | Start: 1515 |
| Tm | 59.969 | 59.962 | End: 1710 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
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| Sequence | GATCCGAGTCTGCTCTCTGC | AACTGCGTACCTTCAGACGG | Start: 1515 |
| Tm | 59.969 | 60.039 | End: 1772 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
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| Sequence | TAACTTCGGGCTAGCTCCCT | TAGCTGCTCTAGTGGGGAGG | Start: 893 |
| Tm | 60.032 | 60.106 | End: 1070 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
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| Sequence | TAACTTCGGGCTAGCTCCCT | TGCTCTAGTGGGGAGGTCAA | Start: 893 |
| Tm | 60.032 | 59.884 | End: 1066 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
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| Sequence | GTAACTTCGGGCTAGCTCCC | TAGCTGCTCTAGTGGGGAGG | Start: 892 |
| Tm | 59.896 | 60.106 | End: 1070 |
| Size | 20 | 20 | 179 |
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