Primer Details for TRINITY_DN67045_c3_g1_i1 |
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| (T)19(G)10acaatattattacaa(TAT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGTACAAAATTGTCTGAATTTTT | CCTGTACTGCCGTACCAAGG | Start: 62 |
| Tm | 57.237 | 60.108 | End: 234 |
| Size | 25 | 20 | 173 |
| Primer 2 details |
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| Sequence | TTGCGTACAAAATTGTCTGAATTTT | CCTGTACTGCCGTACCAAGG | Start: 61 |
| Tm | 57.237 | 60.108 | End: 234 |
| Size | 25 | 20 | 174 |
| Primer 3 details |
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| Sequence | TGCGTACAAAATTGTCTGAATTTTT | TGAACCTTCAGACGTTGCCA | Start: 62 |
| Tm | 57.237 | 59.821 | End: 202 |
| Size | 25 | 20 | 141 |
| Primer 4 details |
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| Sequence | TTGCGTACAAAATTGTCTGAATTTT | TGAACCTTCAGACGTTGCCA | Start: 61 |
| Tm | 57.237 | 59.821 | End: 202 |
| Size | 25 | 20 | 142 |
| Primer 5 details |
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| Sequence | TGTATTTTTGACGCAATTTAAGCAT | CCTGTACTGCCGTACCAAGG | Start: 0 |
| Tm | 57.118 | 60.108 | End: 234 |
| Size | 25 | 20 | 235 |
