ssr_primer
Primer Details for TRINITY_DN67002_c5_g4_i1 |
|---|
| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGTTCGGAATGGCTCAGG | TTAGACGGAAGGGGGACGAT | Start: 618 |
| Tm | 60.036 | 60.033 | End: 822 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
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| Sequence | ATCGTCCCCCTTCCGTCTAA | ACACGATGGCAGTGACGAAT | Start: 803 |
| Tm | 60.033 | 60.037 | End: 1079 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GACCACTGGGAAATGGCTGA | TTAGACGGAAGGGGGACGAT | Start: 644 |
| Tm | 59.962 | 60.033 | End: 822 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | ATTCGTCACTGCCATCGTGT | TAAAAGCAGGCACCGCTACA | Start: 1060 |
| Tm | 60.037 | 59.964 | End: 1194 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | ATTCGTCACTGCCATCGTGT | CTGCCAACTGCCAACTCCTA | Start: 1060 |
| Tm | 60.037 | 59.963 | End: 1253 |
| Size | 20 | 20 | 194 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGTTCGGAATGGCTCAGG | TTAGACGGAAGGGGGACGAT | Start: 618 |
| Tm | 60.036 | 60.033 | End: 822 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | ATCGTCCCCCTTCCGTCTAA | ACACGATGGCAGTGACGAAT | Start: 803 |
| Tm | 60.033 | 60.037 | End: 1079 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GACCACTGGGAAATGGCTGA | TTAGACGGAAGGGGGACGAT | Start: 644 |
| Tm | 59.962 | 60.033 | End: 822 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | ATTCGTCACTGCCATCGTGT | TAAAAGCAGGCACCGCTACA | Start: 1060 |
| Tm | 60.037 | 59.964 | End: 1194 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | ATTCGTCACTGCCATCGTGT | CTGCCAACTGCCAACTCCTA | Start: 1060 |
| Tm | 60.037 | 59.963 | End: 1253 |
| Size | 20 | 20 | 194 |
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