ssr_primer
Primer Details for TRINITY_DN66961_c5_g5_i4 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTAGCAAAAGAGGGCCAGA | TTCGCTCGCCCCCTTAATTT | Start: 659 |
| Tm | 60.036 | 60.035 | End: 758 |
| Size | 20 | 20 | 100 |
| Primer 2 details |
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| Sequence | CGTAGCAAAAGAGGGCCAGA | TTTCGCTCGCCCCCTTAATT | Start: 659 |
| Tm | 60.036 | 60.035 | End: 759 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
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| Sequence | CGTAGCAAAAGAGGGCCAGA | TTTTCGCTCGCCCCCTTAAT | Start: 659 |
| Tm | 60.036 | 60.035 | End: 760 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
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| Sequence | GGCACTAACCGGGGATCAAA | TCTGGCCCTCTTTTGCTACG | Start: 433 |
| Tm | 60.035 | 60.036 | End: 678 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
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| Sequence | CGTAGCAAAAGAGGGCCAGA | GTCTGCTCCTCAACCTCACC | Start: 659 |
| Tm | 60.036 | 60.037 | End: 788 |
| Size | 20 | 20 | 130 |
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