ssr_primer
Primer Details for TRINITY_DN66955_c2_g2_i8 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTCTGCAATAAGGCGCCA | GTCACCATGGCCGAGAATGA | Start: 478 |
| Tm | 60.036 | 60.108 | End: 629 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | GGCAGCTTCTCTTCTGACGT | GTCACCATGGCCGAGAATGA | Start: 498 |
| Tm | 60.038 | 60.108 | End: 629 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | TGTTGAGTGCGTTTCTCCCA | GGATTGCATACACAGCCCCT | Start: 1299 |
| Tm | 59.821 | 60.107 | End: 1442 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | AGGCAGCTTCTCTTCTGACG | GTCACCATGGCCGAGAATGA | Start: 497 |
| Tm | 59.754 | 60.108 | End: 629 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | TTCTCTGCAATAAGGCGCCA | TTGTCACCATGGCCGAGAAT | Start: 478 |
| Tm | 60.036 | 59.672 | End: 631 |
| Size | 20 | 20 | 154 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTCTGCAATAAGGCGCCA | GTCACCATGGCCGAGAATGA | Start: 478 |
| Tm | 60.036 | 60.108 | End: 629 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | GGCAGCTTCTCTTCTGACGT | GTCACCATGGCCGAGAATGA | Start: 498 |
| Tm | 60.038 | 60.108 | End: 629 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | TGTTGAGTGCGTTTCTCCCA | GGATTGCATACACAGCCCCT | Start: 1299 |
| Tm | 59.821 | 60.107 | End: 1442 |
| Size | 20 | 20 | 144 |
| Primer 4 details |
|---|
| Sequence | AGGCAGCTTCTCTTCTGACG | GTCACCATGGCCGAGAATGA | Start: 497 |
| Tm | 59.754 | 60.108 | End: 629 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | TTCTCTGCAATAAGGCGCCA | TTGTCACCATGGCCGAGAAT | Start: 478 |
| Tm | 60.036 | 59.672 | End: 631 |
| Size | 20 | 20 | 154 |
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