ssr_primer
Primer Details for TRINITY_DN66865_c3_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGGCATGTAACCGTCCTT | TGTGGGTCGCTCGCATAATT | Start: 612 |
| Tm | 60.322 | 60.108 | End: 783 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
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| Sequence | GGTGGCATGTAACCGTCCTT | TGGGTCGCTCGCATAATTCA | Start: 612 |
| Tm | 60.322 | 59.823 | End: 781 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
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| Sequence | CAAAGTCCCCGGCTTCAAGA | AAGGACGGTTACATGCCACC | Start: 447 |
| Tm | 60.251 | 60.322 | End: 631 |
| Size | 20 | 20 | 185 |
| Primer 4 details |
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| Sequence | AAGACAAAGTCCCCGGCTTC | AAGGACGGTTACATGCCACC | Start: 443 |
| Tm | 60.251 | 60.322 | End: 631 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
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| Sequence | TTTTGACTACTCGTGGCCCG | AAGGACGGTTACATGCCACC | Start: 468 |
| Tm | 60.32 | 60.322 | End: 631 |
| Size | 20 | 20 | 164 |
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