ssr_primer
Primer Details for TRINITY_DN66857_c4_g1_i4 |
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| (A)17gaggaggcagtagtttttta(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGTTCCTCGGGGCAAAGA | CCCTCCACGTCTCCTGAGTA | Start: 1153 |
| Tm | 59.964 | 60.034 | End: 1371 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
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| Sequence | CCTTTCTCTTTCGGGGGCAT | TGGAAGAGAGAAAGCTGCCG | Start: 1867 |
| Tm | 60.034 | 60.037 | End: 2003 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
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| Sequence | CGCCCACTTCCCATCTCTTT | TGGAAGAGAGAAAGCTGCCG | Start: 1892 |
| Tm | 60.035 | 60.037 | End: 2003 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
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| Sequence | CCATCTCTTTCACGCACCCT | TGGAAGAGAGAAAGCTGCCG | Start: 1902 |
| Tm | 60.036 | 60.037 | End: 2003 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
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| Sequence | TCCCATCTCTTTCACGCACC | TGGAAGAGAGAAAGCTGCCG | Start: 1900 |
| Tm | 60.036 | 60.037 | End: 2003 |
| Size | 20 | 20 | 104 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGTTCCTCGGGGCAAAGA | CCCTCCACGTCTCCTGAGTA | Start: 1153 |
| Tm | 59.964 | 60.034 | End: 1371 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | CCTTTCTCTTTCGGGGGCAT | TGGAAGAGAGAAAGCTGCCG | Start: 1867 |
| Tm | 60.034 | 60.037 | End: 2003 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | CGCCCACTTCCCATCTCTTT | TGGAAGAGAGAAAGCTGCCG | Start: 1892 |
| Tm | 60.035 | 60.037 | End: 2003 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | CCATCTCTTTCACGCACCCT | TGGAAGAGAGAAAGCTGCCG | Start: 1902 |
| Tm | 60.036 | 60.037 | End: 2003 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | TCCCATCTCTTTCACGCACC | TGGAAGAGAGAAAGCTGCCG | Start: 1900 |
| Tm | 60.036 | 60.037 | End: 2003 |
| Size | 20 | 20 | 104 |
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