ssr_primer
Primer Details for TRINITY_DN66829_c1_g1_i4 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGCGGATGGTGCTGAAAT | ATGTCAACACCGAGCGAACT | Start: 1760 |
| Tm | 60.035 | 59.966 | End: 1994 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
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| Sequence | TAGCGTGGGTTGTGATGTCC | GTCCTCCTGACAACCCACAG | Start: 1233 |
| Tm | 60.037 | 59.965 | End: 1430 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
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| Sequence | CAATCTGCTGTCGTTGAGCG | ATGTCAACACCGAGCGAACT | Start: 1746 |
| Tm | 59.906 | 59.966 | End: 1994 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
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| Sequence | TCCCAGAACAGACCTTCCCA | GGTGGACCCATCAACGTCAT | Start: 3805 |
| Tm | 60.105 | 60.035 | End: 3955 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
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| Sequence | AGTTCGCTCGGTGTTGACAT | AGGAATGTTGGATCCGCTGG | Start: 1975 |
| Tm | 59.966 | 60.107 | End: 2227 |
| Size | 20 | 20 | 253 |
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