ssr_primer
Primer Details for TRINITY_DN66816_c6_g1_i4 |
|---|
| (G)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTTTTACGGCTGTGCTGC | TGACAGGAACAATGACCCCG | Start: 1374 |
| Tm | 59.97 | 59.964 | End: 1478 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | GGGCATTGTTTGTCGGCAAT | GCAGCACAGCCGTAAAAACA | Start: 1159 |
| Tm | 60.037 | 59.97 | End: 1393 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | GGGCATTGTTTGTCGGCAAT | TACAGCAGCACAGCCGTAAA | Start: 1159 |
| Tm | 60.037 | 59.966 | End: 1397 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | GGGCATTGTTTGTCGGCAAT | GCATTGTTGCCACTACCACG | Start: 1159 |
| Tm | 60.037 | 60.11 | End: 1342 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | TCCACAATTTGCCAGAGGGG | GCAGCACAGCCGTAAAAACA | Start: 1185 |
| Tm | 60.252 | 59.97 | End: 1393 |
| Size | 20 | 20 | 209 |
(C)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTTTTACGGCTGTGCTGC | TGACAGGAACAATGACCCCG | Start: 1374 |
| Tm | 59.97 | 59.964 | End: 1478 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
|---|
| Sequence | GGGCATTGTTTGTCGGCAAT | GCAGCACAGCCGTAAAAACA | Start: 1159 |
| Tm | 60.037 | 59.97 | End: 1393 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | GGGCATTGTTTGTCGGCAAT | TACAGCAGCACAGCCGTAAA | Start: 1159 |
| Tm | 60.037 | 59.966 | End: 1397 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | GGGCATTGTTTGTCGGCAAT | GCATTGTTGCCACTACCACG | Start: 1159 |
| Tm | 60.037 | 60.11 | End: 1342 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | TCCACAATTTGCCAGAGGGG | GCAGCACAGCCGTAAAAACA | Start: 1185 |
| Tm | 60.252 | 59.97 | End: 1393 |
| Size | 20 | 20 | 209 |
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