ssr_primer
Primer Details for TRINITY_DN66815_c2_g1_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGCGTACTGGCAGGAAG | CCCAAAGCAAAGCACCCTTC | Start: 647 |
| Tm | 60.037 | 59.966 | End: 798 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | TGGTCACAGTTGTCGCAAGT | CCCAAAGCAAAGCACCCTTC | Start: 526 |
| Tm | 60.108 | 59.966 | End: 798 |
| Size | 20 | 20 | 273 |
| Primer 3 details |
|---|
| Sequence | GAAGGGTGCTTTGCTTTGGG | TTCTGGACTTGGCACCACTC | Start: 779 |
| Tm | 59.966 | 59.891 | End: 937 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | TGGTCACAGTTGTCGCAAGT | CTTCCTGCCAGTACGCAAGA | Start: 526 |
| Tm | 60.108 | 60.037 | End: 666 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | GCGTGTCATCTGCTAGGTGA | CTTCCTGCCAGTACGCAAGA | Start: 431 |
| Tm | 59.827 | 60.037 | End: 666 |
| Size | 20 | 20 | 236 |
(G)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGCGTACTGGCAGGAAG | CCCAAAGCAAAGCACCCTTC | Start: 647 |
| Tm | 60.037 | 59.966 | End: 798 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | TGGTCACAGTTGTCGCAAGT | CCCAAAGCAAAGCACCCTTC | Start: 526 |
| Tm | 60.108 | 59.966 | End: 798 |
| Size | 20 | 20 | 273 |
| Primer 3 details |
|---|
| Sequence | GAAGGGTGCTTTGCTTTGGG | TTCTGGACTTGGCACCACTC | Start: 779 |
| Tm | 59.966 | 59.891 | End: 937 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
|---|
| Sequence | TGGTCACAGTTGTCGCAAGT | CTTCCTGCCAGTACGCAAGA | Start: 526 |
| Tm | 60.108 | 60.037 | End: 666 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | GCGTGTCATCTGCTAGGTGA | CTTCCTGCCAGTACGCAAGA | Start: 431 |
| Tm | 59.827 | 60.037 | End: 666 |
| Size | 20 | 20 | 236 |
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