ssr_primer
Primer Details for TRINITY_DN66815_c0_g1_i1 |
|---|
| (G)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGTCCTCCAGAGAAGTA | AGACGAGACGCACCAAAACT | Start: 104 |
| Tm | 59.661 | 59.896 | End: 345 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | AAACAAGACCACACCCTCCC | TACTTCTCTGGAGGACCCCC | Start: 11 |
| Tm | 59.815 | 59.661 | End: 123 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | GGGGGTCCTCCAGAGAAGTA | ACGCACCAAAACTAGCCGAT | Start: 104 |
| Tm | 59.661 | 60.321 | End: 338 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | CACACCCTCCCCATTCCAAG | TACTTCTCTGGAGGACCCCC | Start: 20 |
| Tm | 60.324 | 59.661 | End: 123 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | GGGGGTCCTCCAGAGAAGTA | CGAGACGCACCAAAACTAGC | Start: 104 |
| Tm | 59.661 | 59.561 | End: 342 |
| Size | 20 | 20 | 239 |
(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGTCCTCCAGAGAAGTA | AGACGAGACGCACCAAAACT | Start: 104 |
| Tm | 59.661 | 59.896 | End: 345 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | AAACAAGACCACACCCTCCC | TACTTCTCTGGAGGACCCCC | Start: 11 |
| Tm | 59.815 | 59.661 | End: 123 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | GGGGGTCCTCCAGAGAAGTA | ACGCACCAAAACTAGCCGAT | Start: 104 |
| Tm | 59.661 | 60.321 | End: 338 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | CACACCCTCCCCATTCCAAG | TACTTCTCTGGAGGACCCCC | Start: 20 |
| Tm | 60.324 | 59.661 | End: 123 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | GGGGGTCCTCCAGAGAAGTA | CGAGACGCACCAAAACTAGC | Start: 104 |
| Tm | 59.661 | 59.561 | End: 342 |
| Size | 20 | 20 | 239 |
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