Primer Details for TRINITY_DN66812_c15_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGAGTGGGCGGAAAATGG | GCAGATCCTGAGAATAACAATGAAGG | Start: 61 |
| Tm | 60.035 | 60.017 | End: 231 |
| Size | 20 | 26 | 171 |
| Primer 2 details |
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| Sequence | AATGGGTCGAGAAGCACTGG | GCAGATCCTGAGAATAACAATGAAGG | Start: 76 |
| Tm | 60.036 | 60.017 | End: 231 |
| Size | 20 | 26 | 156 |
| Primer 3 details |
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| Sequence | CGAGAAGCACTGGCCCATAA | GCAGATCCTGAGAATAACAATGAAGG | Start: 83 |
| Tm | 60.108 | 60.017 | End: 231 |
| Size | 20 | 26 | 149 |
| Primer 4 details |
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| Sequence | AGAGAGTGGGCGGAAAATGG | AGCAGATCCTGAGAATAACAATGA | Start: 61 |
| Tm | 60.035 | 57.755 | End: 232 |
| Size | 20 | 24 | 172 |
| Primer 5 details |
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| Sequence | AATGGGTCGAGAAGCACTGG | AGCAGATCCTGAGAATAACAATGA | Start: 76 |
| Tm | 60.036 | 57.755 | End: 232 |
| Size | 20 | 24 | 157 |
