ssr_primer
Primer Details for TRINITY_DN66812_c11_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAGTTAGGCGTGCAATGT | TGGTCAAAAGAACGTGTTTGGA | Start: 122 |
| Tm | 60.038 | 58.917 | End: 223 |
| Size | 20 | 22 | 102 |
| Primer 2 details |
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| Sequence | TCATCGAGTTAGGCGTGCAA | TGGTCAAAAGAACGTGTTTGGA | Start: 119 |
| Tm | 59.755 | 58.917 | End: 223 |
| Size | 20 | 22 | 105 |
| Primer 3 details |
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| Sequence | TCGAGTTAGGCGTGCAATGT | TGGTCAAAAGAACGTGTTTGG | Start: 122 |
| Tm | 60.038 | 57.504 | End: 223 |
| Size | 20 | 21 | 102 |
| Primer 4 details |
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| Sequence | ATCATCGAGTTAGGCGTGCA | TGGTCAAAAGAACGTGTTTGGA | Start: 118 |
| Tm | 59.54 | 58.917 | End: 223 |
| Size | 20 | 22 | 106 |
| Primer 5 details |
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| Sequence | TCGAGTTAGGCGTGCAATGT | TGGTCAAAAGAACGTGTTTGGAA | Start: 122 |
| Tm | 60.038 | 59.494 | End: 223 |
| Size | 20 | 23 | 102 |
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