Primer Details for TRINITY_DN66805_c8_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGACAATTTTTCCCAGTGA | TCAGACAGTTTTGTTATCAATTTTCTC | Start: 53 |
| Tm | 58.453 | 57.049 | End: 176 |
| Size | 22 | 27 | 124 |
| Primer 2 details |
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| Sequence | TGACGACAATTTTTCCCAGTGA | CAGACAGTTTTGTTATCAATTTTCTCT | Start: 53 |
| Tm | 58.453 | 57.043 | End: 175 |
| Size | 22 | 27 | 123 |
| Primer 3 details |
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| Sequence | TGACGACAATTTTTCCCAGTGAA | TCAGACAGTTTTGTTATCAATTTTCTC | Start: 53 |
| Tm | 59.052 | 57.049 | End: 176 |
| Size | 23 | 27 | 124 |
| Primer 4 details |
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| Sequence | TGACGACAATTTTTCCCAGTGAA | CAGACAGTTTTGTTATCAATTTTCTCT | Start: 53 |
| Tm | 59.052 | 57.043 | End: 175 |
| Size | 23 | 27 | 123 |
| Primer 5 details |
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| Sequence | TGACGACAATTTTTCCCAGTG | TCAGACAGTTTTGTTATCAATTTTCTC | Start: 53 |
| Tm | 57.019 | 57.049 | End: 176 |
| Size | 21 | 27 | 124 |
