ssr_primer
Primer Details for TRINITY_DN66782_c3_g1_i5 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAAGGTTTCGGCGTTTGC | TCGTTCTGCCGCTTGTAGTT | Start: 119 |
| Tm | 59.97 | 59.967 | End: 398 |
| Size | 20 | 20 | 280 |
| Primer 2 details |
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| Sequence | CCGTCTCCATCGTCGAGAAG | CGTGGATGAAGTCAAGCCCT | Start: 44 |
| Tm | 59.972 | 60.036 | End: 163 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
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| Sequence | CCGTCTCCATCGTCGAGAAG | TCGTGGATGAAGTCAAGCCC | Start: 44 |
| Tm | 59.972 | 60.036 | End: 164 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | AGAAAGGTTTCGGCGTTTGC | GGTGTTGGAAGACTGGGAGG | Start: 119 |
| Tm | 59.97 | 59.963 | End: 358 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
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| Sequence | GTCTAACAGTGTGGCCGACA | CTGAGACAAAGCAGGAGCCA | Start: 870 |
| Tm | 59.967 | 59.964 | End: 1010 |
| Size | 20 | 20 | 141 |
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