ssr_primer
Primer Details for TRINITY_DN66753_c0_g2_i1 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACGTTCATCCACTCTCCA | ACGCAGCGTTCTTCAGAGAA | Start: 520 |
| Tm | 60.037 | 59.968 | End: 779 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
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| Sequence | AGTGGCCCCAGTAACATGTG | TCCTCAACTGACAGCGCAAT | Start: 297 |
| Tm | 59.962 | 59.965 | End: 472 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
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| Sequence | AGTGAGGACTGAAGCAAGGC | CACATGTTACTGGGGCCACT | Start: 115 |
| Tm | 59.964 | 59.962 | End: 316 |
| Size | 20 | 20 | 202 |
| Primer 4 details |
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| Sequence | AGTGGCCCCAGTAACATGTG | TGGAGAGTGGATGAACGTGC | Start: 297 |
| Tm | 59.962 | 60.037 | End: 539 |
| Size | 20 | 20 | 243 |
| Primer 5 details |
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| Sequence | ACCAGGATCGGATTTGCAGG | TCCTCAACTGACAGCGCAAT | Start: 369 |
| Tm | 60.107 | 59.965 | End: 472 |
| Size | 20 | 20 | 104 |
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