ssr_primer
Primer Details for TRINITY_DN66731_c3_g2_i3 |
|---|
| (T)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCGCGCTTTCAGCAAAAA | TCGTGGTTCGTTGAGGGAAG | Start: 1220 |
| Tm | 59.899 | 59.967 | End: 1421 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | AAAATTGTCGGGTAGGCGGT | CATGAACCGATCTGGCCGTA | Start: 1872 |
| Tm | 59.963 | 59.896 | End: 1978 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | AAAATTGTCGGGTAGGCGGT | ATGAACCGATCTGGCCGTAC | Start: 1872 |
| Tm | 59.963 | 59.896 | End: 1977 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | CGGTCGACAGCAGAATGTCT | ATTCTGCAAGGGTAGGTGCC | Start: 958 |
| Tm | 60.109 | 60.034 | End: 1091 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | CTCGGTCGACAGCAGAATGT | ATTCTGCAAGGGTAGGTGCC | Start: 956 |
| Tm | 60.109 | 60.034 | End: 1091 |
| Size | 20 | 20 | 136 |
(T)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCGCGCTTTCAGCAAAAA | TCGTGGTTCGTTGAGGGAAG | Start: 1220 |
| Tm | 59.899 | 59.967 | End: 1421 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | AAAATTGTCGGGTAGGCGGT | CATGAACCGATCTGGCCGTA | Start: 1872 |
| Tm | 59.963 | 59.896 | End: 1978 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | AAAATTGTCGGGTAGGCGGT | ATGAACCGATCTGGCCGTAC | Start: 1872 |
| Tm | 59.963 | 59.896 | End: 1977 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | CGGTCGACAGCAGAATGTCT | ATTCTGCAAGGGTAGGTGCC | Start: 958 |
| Tm | 60.109 | 60.034 | End: 1091 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | CTCGGTCGACAGCAGAATGT | ATTCTGCAAGGGTAGGTGCC | Start: 956 |
| Tm | 60.109 | 60.034 | End: 1091 |
| Size | 20 | 20 | 136 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCGCGCTTTCAGCAAAAA | TCGTGGTTCGTTGAGGGAAG | Start: 1220 |
| Tm | 59.899 | 59.967 | End: 1421 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | AAAATTGTCGGGTAGGCGGT | CATGAACCGATCTGGCCGTA | Start: 1872 |
| Tm | 59.963 | 59.896 | End: 1978 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | AAAATTGTCGGGTAGGCGGT | ATGAACCGATCTGGCCGTAC | Start: 1872 |
| Tm | 59.963 | 59.896 | End: 1977 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | CGGTCGACAGCAGAATGTCT | ATTCTGCAAGGGTAGGTGCC | Start: 958 |
| Tm | 60.109 | 60.034 | End: 1091 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
|---|
| Sequence | CTCGGTCGACAGCAGAATGT | ATTCTGCAAGGGTAGGTGCC | Start: 956 |
| Tm | 60.109 | 60.034 | End: 1091 |
| Size | 20 | 20 | 136 |
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