Primer Details for TRINITY_DN66726_c7_g2_i1 |
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| (A)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTGAACCAAATGGAGCCT | CCGTTGAAGTTTACTAGAGATTTGCA | Start: 68 |
| Tm | 59.89 | 59.853 | End: 206 |
| Size | 20 | 26 | 139 |
| Primer 2 details |
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| Sequence | ACGTGAACCAAATGGAGCCT | ACCGTTGAAGTTTACTAGAGATTTGC | Start: 68 |
| Tm | 59.89 | 59.853 | End: 207 |
| Size | 20 | 26 | 140 |
| Primer 3 details |
|---|
| Sequence | ACGTGAACCAAATGGAGCCT | CCGTTGAAGTTTACTAGAGATTTGC | Start: 68 |
| Tm | 59.89 | 58.465 | End: 206 |
| Size | 20 | 25 | 139 |
| Primer 4 details |
|---|
| Sequence | AACCAAATGGAGCCTCCTGC | CCGTTGAAGTTTACTAGAGATTTGCA | Start: 73 |
| Tm | 60.613 | 59.853 | End: 206 |
| Size | 20 | 26 | 134 |
| Primer 5 details |
|---|
| Sequence | AACCAAATGGAGCCTCCTGC | ACCGTTGAAGTTTACTAGAGATTTGC | Start: 73 |
| Tm | 60.613 | 59.853 | End: 207 |
| Size | 20 | 26 | 135 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTGAACCAAATGGAGCCT | CCGTTGAAGTTTACTAGAGATTTGCA | Start: 68 |
| Tm | 59.89 | 59.853 | End: 206 |
| Size | 20 | 26 | 139 |
| Primer 2 details |
|---|
| Sequence | ACGTGAACCAAATGGAGCCT | ACCGTTGAAGTTTACTAGAGATTTGC | Start: 68 |
| Tm | 59.89 | 59.853 | End: 207 |
| Size | 20 | 26 | 140 |
| Primer 3 details |
|---|
| Sequence | ACGTGAACCAAATGGAGCCT | CCGTTGAAGTTTACTAGAGATTTGC | Start: 68 |
| Tm | 59.89 | 58.465 | End: 206 |
| Size | 20 | 25 | 139 |
| Primer 4 details |
|---|
| Sequence | AACCAAATGGAGCCTCCTGC | CCGTTGAAGTTTACTAGAGATTTGCA | Start: 73 |
| Tm | 60.613 | 59.853 | End: 206 |
| Size | 20 | 26 | 134 |
| Primer 5 details |
|---|
| Sequence | AACCAAATGGAGCCTCCTGC | ACCGTTGAAGTTTACTAGAGATTTGC | Start: 73 |
| Tm | 60.613 | 59.853 | End: 207 |
| Size | 20 | 26 | 135 |
