ssr_primer
Primer Details for TRINITY_DN66721_c4_g5_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGATTGTTGCGTTTCCTT | TGGCCACTTGTGATACGAGG | Start: 2354 |
| Tm | 60.04 | 59.751 | End: 2513 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
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| Sequence | GCGGATTGTTGCGTTTCCTT | GGCCACTTGTGATACGAGGT | Start: 2354 |
| Tm | 60.04 | 59.751 | End: 2512 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
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| Sequence | ATGCGGATTGTTGCGTTTCC | TGGCCACTTGTGATACGAGG | Start: 2352 |
| Tm | 60.109 | 59.751 | End: 2513 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | ATGCGGATTGTTGCGTTTCC | GGCCACTTGTGATACGAGGT | Start: 2352 |
| Tm | 60.109 | 59.751 | End: 2512 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
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| Sequence | TTTACACGGACCGCTGGAAG | GCAGCGAAAAACGAAGCTCA | Start: 1088 |
| Tm | 60.32 | 60.041 | End: 1299 |
| Size | 20 | 20 | 212 |
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