ssr_primer
Primer Details for TRINITY_DN66717_c2_g1_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTGTTGGGGGCAGAAGGT | CCCCCATTTGGAACTGACGA | Start: 517 |
| Tm | 60.032 | 59.962 | End: 618 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
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| Sequence | TTTTGTTGGGGGCAGAAGGT | AATTTCTCCGGCCCCCATTT | Start: 517 |
| Tm | 60.032 | 59.957 | End: 629 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | CGTAACTGCGATCAATGCCG | ACCTTCTGCCCCCAACAAAA | Start: 318 |
| Tm | 60.043 | 60.032 | End: 536 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
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| Sequence | GGACTCGACGTAACTGCGAT | ACCTTCTGCCCCCAACAAAA | Start: 310 |
| Tm | 59.902 | 60.032 | End: 536 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
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| Sequence | TGCGATCAATGCCGAGATGA | ACCTTCTGCCCCCAACAAAA | Start: 324 |
| Tm | 59.895 | 60.032 | End: 536 |
| Size | 20 | 20 | 213 |
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