Primer Details for TRINITY_DN66689_c2_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAAGAAAAAGGAAAGAAAGACGT | AAAGTTTTGTTTTCAGGGCTTTTT | Start: 59 |
| Tm | 57.847 | 57.107 | End: 265 |
| Size | 25 | 24 | 207 |
| Primer 2 details |
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| Sequence | AGAATAGAGTTCGAGGTTAAAAGGA | AAAGTTTTGTTTTCAGGGCTTTTT | Start: 114 |
| Tm | 57.522 | 57.107 | End: 265 |
| Size | 25 | 24 | 152 |
| Primer 3 details |
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| Sequence | TTGAAAGAAAAAGGAAAGAAAGACGT | AAAGTTTTGTTTTCAGGGCTTTTT | Start: 58 |
| Tm | 58.401 | 57.107 | End: 265 |
| Size | 26 | 24 | 208 |
| Primer 4 details |
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| Sequence | TGAAAGAAAAAGGAAAGAAAGACGT | CTAAAGTTTTGTTTTCAGGGCTTTT | Start: 59 |
| Tm | 57.847 | 57.39 | End: 267 |
| Size | 25 | 25 | 209 |
| Primer 5 details |
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| Sequence | AGAATAGAGTTCGAGGTTAAAAGGAA | AAAGTTTTGTTTTCAGGGCTTTTT | Start: 114 |
| Tm | 58.096 | 57.107 | End: 265 |
| Size | 26 | 24 | 152 |
