ssr_primer
Primer Details for TRINITY_DN66689_c1_g5_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCTCATGTCCTTCGCATT | AGCAAAGTGGAAAAGGGGGT | Start: 1359 |
| Tm | 60.107 | 59.735 | End: 1507 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
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| Sequence | ACCCCCTTTTCCACTTTGCT | GTCACTAGCACGCGGTAAGT | Start: 1488 |
| Tm | 59.735 | 60.109 | End: 1704 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
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| Sequence | CCCGCAATTTTGTTGCAAGC | AGATCTCAGGAAGCACCCGA | Start: 2202 |
| Tm | 59.765 | 60.325 | End: 2309 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
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| Sequence | ACCCCCTTTTCCACTTTGCT | TCAACTGGTCACTAGCACGC | Start: 1488 |
| Tm | 59.735 | 60.32 | End: 1711 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
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| Sequence | ACAAGCCTGTGATAACCCCC | AGCAAAGTGGAAAAGGGGGT | Start: 1337 |
| Tm | 59.668 | 59.735 | End: 1507 |
| Size | 20 | 20 | 171 |
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