Primer Details for TRINITY_DN66671_c6_g1_i1 |
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| (AAAT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGATAGTGGTTCAATTATCGTAGGA | TTGACGTCCTTGATAGGCACA | Start: 29 |
| Tm | 59.512 | 59.379 | End: 128 |
| Size | 26 | 21 | 100 |
| Primer 2 details |
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| Sequence | TTCGATAGTGGTTCAATTATCGTAGGA | TGACGTCCTTGATAGGCACA | Start: 28 |
| Tm | 59.986 | 58.738 | End: 127 |
| Size | 27 | 20 | 100 |
| Primer 3 details |
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| Sequence | TCGATAGTGGTTCAATTATCGTAGG | TTGACGTCCTTGATAGGCACA | Start: 29 |
| Tm | 58.325 | 59.379 | End: 128 |
| Size | 25 | 21 | 100 |
| Primer 4 details |
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| Sequence | TTCGATAGTGGTTCAATTATCGTAGG | TGACGTCCTTGATAGGCACA | Start: 28 |
| Tm | 58.857 | 58.738 | End: 127 |
| Size | 26 | 20 | 100 |
| Primer 5 details |
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| Sequence | TCGATAGTGGTTCAATTATCGTAGGA | TTGACGTCCTTGATAGGCACAA | Start: 29 |
| Tm | 59.512 | 59.962 | End: 128 |
| Size | 26 | 22 | 100 |
