ssr_primer
Primer Details for TRINITY_DN66669_c1_g1_i1 |
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| (T)21ccgcgggttcgcgcgcttttgccgtaaacctttttttctggtgtaggggaagcccctttt(C)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTTCAATGCCGCCGATGAG | TTTTGCAGCTCGCTTGACAC | Start: 382 |
| Tm | 59.971 | 59.971 | End: 645 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
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| Sequence | CTCTTTCAATGCCGCCGATG | TTTTGCAGCTCGCTTGACAC | Start: 380 |
| Tm | 59.971 | 59.971 | End: 645 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
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| Sequence | CTAAAAATACGTGCCCCGCG | ACTTTTTGGCTGGGGTGACA | Start: 914 |
| Tm | 59.973 | 60.033 | End: 1050 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
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| Sequence | CTAAAAATACGTGCCCCGCG | CAAGTACGCCCCTCCACTTT | Start: 914 |
| Tm | 59.973 | 59.964 | End: 1065 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
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| Sequence | GGTAGTTTGGCAGACTCGCT | CATCGGCGGCATTGAAAGAG | Start: 182 |
| Tm | 60.037 | 59.971 | End: 399 |
| Size | 20 | 20 | 218 |
(A)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTTCAATGCCGCCGATGAG | TTTTGCAGCTCGCTTGACAC | Start: 382 |
| Tm | 59.971 | 59.971 | End: 645 |
| Size | 20 | 20 | 264 |
| Primer 2 details |
|---|
| Sequence | CTCTTTCAATGCCGCCGATG | TTTTGCAGCTCGCTTGACAC | Start: 380 |
| Tm | 59.971 | 59.971 | End: 645 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | CTAAAAATACGTGCCCCGCG | ACTTTTTGGCTGGGGTGACA | Start: 914 |
| Tm | 59.973 | 60.033 | End: 1050 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | CTAAAAATACGTGCCCCGCG | CAAGTACGCCCCTCCACTTT | Start: 914 |
| Tm | 59.973 | 59.964 | End: 1065 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
|---|
| Sequence | GGTAGTTTGGCAGACTCGCT | CATCGGCGGCATTGAAAGAG | Start: 182 |
| Tm | 60.037 | 59.971 | End: 399 |
| Size | 20 | 20 | 218 |
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