ssr_primer
Primer Details for TRINITY_DN66646_c2_g1_i3 |
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| (T)16ccagtgtgggggtttttacgccgttacaaagccgtgtattgatcgaggtgtgcgaaatggatttggt(C)10(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAACAGACTCGGTTGCAT | GGAAGGAGCCATGTAGAGCC | Start: 344 |
| Tm | 59.966 | 59.892 | End: 505 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
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| Sequence | ACGGTTGCCACAACCTAGAG | GGAAGGAGCCATGTAGAGCC | Start: 375 |
| Tm | 59.965 | 59.892 | End: 505 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
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| Sequence | TGGGGGTTTTTACGCCGTTA | ATGCAACCGAGTCTGTTCGT | Start: 88 |
| Tm | 59.891 | 59.966 | End: 363 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
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| Sequence | GCTCTCTCGGTTCTGGGAAC | CTCTAGGTTGTGGCAACCGT | Start: 278 |
| Tm | 60.109 | 59.965 | End: 394 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
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| Sequence | TTCACGTAACCGCTCTCTCG | CTCTAGGTTGTGGCAACCGT | Start: 267 |
| Tm | 59.833 | 59.965 | End: 394 |
| Size | 20 | 20 | 128 |
(A)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAACAGACTCGGTTGCAT | GGAAGGAGCCATGTAGAGCC | Start: 344 |
| Tm | 59.966 | 59.892 | End: 505 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
|---|
| Sequence | ACGGTTGCCACAACCTAGAG | GGAAGGAGCCATGTAGAGCC | Start: 375 |
| Tm | 59.965 | 59.892 | End: 505 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | TGGGGGTTTTTACGCCGTTA | ATGCAACCGAGTCTGTTCGT | Start: 88 |
| Tm | 59.891 | 59.966 | End: 363 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | GCTCTCTCGGTTCTGGGAAC | CTCTAGGTTGTGGCAACCGT | Start: 278 |
| Tm | 60.109 | 59.965 | End: 394 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | TTCACGTAACCGCTCTCTCG | CTCTAGGTTGTGGCAACCGT | Start: 267 |
| Tm | 59.833 | 59.965 | End: 394 |
| Size | 20 | 20 | 128 |
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