ssr_primer
Primer Details for TRINITY_DN66594_c3_g3_i1 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCTGCGTTGTTTGATGCA | AACGTGTTCACCTAGCCGAG | Start: 97 |
| Tm | 59.685 | 60.039 | End: 213 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | CTCGGCTAGGTGAACACGTT | GCAAATTTGTGCCCGTCGAA | Start: 194 |
| Tm | 60.039 | 60.318 | End: 391 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CTCGGCTAGGTGAACACGTT | TCCTCAAAGATTGCGTCGCA | Start: 194 |
| Tm | 60.039 | 60.32 | End: 433 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | ATGCTGCGTTGTTTGATGCA | CCGTTTACGTGCGAGAGTCT | Start: 97 |
| Tm | 59.685 | 60.11 | End: 236 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | AGACTCTCGCACGTAAACGG | GCAAATTTGTGCCCGTCGAA | Start: 217 |
| Tm | 60.11 | 60.318 | End: 391 |
| Size | 20 | 20 | 175 |
(A)10(ACAACAACAACAACAACAATTTTACCTAGAAAAGTAATCCTGAGAAAGGAAAAAAAACGATTTTACCCGACTGTAAAATTGTTGGAATTGGTAAGTTTGGAATTTCGACGGGCACAAATTTGCGCTTCGCTGCGTGCATAACTATTGCGACGCAATCTTTGAGGAAAATTTCGAATTTTAGCATATTTTTTCAGCTCTTCTTACGTCAAAAAATTACAGAGAGACGGTTGGTTGCATTAGTTTTCAGAATTTTTACCTGCTTTTCTGAGTTTTTGACAGC)(AAC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGCTGCGTTGTTTGATGCA | AACGTGTTCACCTAGCCGAG | Start: 97 |
| Tm | 59.685 | 60.039 | End: 213 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | CTCGGCTAGGTGAACACGTT | GCAAATTTGTGCCCGTCGAA | Start: 194 |
| Tm | 60.039 | 60.318 | End: 391 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CTCGGCTAGGTGAACACGTT | TCCTCAAAGATTGCGTCGCA | Start: 194 |
| Tm | 60.039 | 60.32 | End: 433 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | ATGCTGCGTTGTTTGATGCA | CCGTTTACGTGCGAGAGTCT | Start: 97 |
| Tm | 59.685 | 60.11 | End: 236 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | AGACTCTCGCACGTAAACGG | GCAAATTTGTGCCCGTCGAA | Start: 217 |
| Tm | 60.11 | 60.318 | End: 391 |
| Size | 20 | 20 | 175 |
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