ssr_primer
Primer Details for TRINITY_DN66552_c1_g1_i4 |
|---|
| (A)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTACCTGACTGCCACGTGA | AACGCAGGAAACTCGTACGT | Start: 819 |
| Tm | 59.967 | 59.969 | End: 1079 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | CTGTTACCTGACTGCCACGT | AACGCAGGAAACTCGTACGT | Start: 817 |
| Tm | 59.966 | 59.969 | End: 1079 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | ATTCCGATCCATCCGTGTCG | GAAGCGTTGAACCCCTCTCA | Start: 174 |
| Tm | 59.968 | 59.965 | End: 275 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
|---|
| Sequence | ATTCCGATCCATCCGTGTCG | TCCACTGAGGAGTCGTCCAT | Start: 174 |
| Tm | 59.968 | 59.96 | End: 370 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | ATAAAGCGCCAGTGACGTCA | GGAGGAATGGAAAAGCGGGA | Start: 1609 |
| Tm | 60.038 | 60.034 | End: 1767 |
| Size | 20 | 20 | 159 |
(T)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTACCTGACTGCCACGTGA | AACGCAGGAAACTCGTACGT | Start: 819 |
| Tm | 59.967 | 59.969 | End: 1079 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | CTGTTACCTGACTGCCACGT | AACGCAGGAAACTCGTACGT | Start: 817 |
| Tm | 59.966 | 59.969 | End: 1079 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | ATTCCGATCCATCCGTGTCG | GAAGCGTTGAACCCCTCTCA | Start: 174 |
| Tm | 59.968 | 59.965 | End: 275 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
|---|
| Sequence | ATTCCGATCCATCCGTGTCG | TCCACTGAGGAGTCGTCCAT | Start: 174 |
| Tm | 59.968 | 59.96 | End: 370 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | ATAAAGCGCCAGTGACGTCA | GGAGGAATGGAAAAGCGGGA | Start: 1609 |
| Tm | 60.038 | 60.034 | End: 1767 |
| Size | 20 | 20 | 159 |
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