Primer Details for TRINITY_DN66551_c4_g1_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTTTTAACTTTTCCCGAAATTTGG | ACCTGGAAAAACTTTTTGATGCT | Start: 48 |
| Tm | 57.573 | 57.642 | End: 161 |
| Size | 26 | 23 | 114 |
| Primer 2 details |
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| Sequence | TTCTTTTAACTTTTCCCGAAATTTGG | AACCTGGAAAAACTTTTTGATGCT | Start: 48 |
| Tm | 57.573 | 58.256 | End: 162 |
| Size | 26 | 24 | 115 |
| Primer 3 details |
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| Sequence | TTTCTTTTAACTTTTCCCGAAATTTGG | ACCTGGAAAAACTTTTTGATGCT | Start: 47 |
| Tm | 58.115 | 57.642 | End: 161 |
| Size | 27 | 23 | 115 |
| Primer 4 details |
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| Sequence | TTCTTTTAACTTTTCCCGAAATTTGG | ACCTGGAAAAACTTTTTGATGCTAGT | Start: 48 |
| Tm | 57.573 | 59.902 | End: 161 |
| Size | 26 | 26 | 114 |
| Primer 5 details |
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| Sequence | TTCTTTTAACTTTTCCCGAAATTTGG | AAACCTGGAAAAACTTTTTGATGCT | Start: 48 |
| Tm | 57.573 | 58.82 | End: 163 |
| Size | 26 | 25 | 116 |
