ssr_primer
Primer Details for TRINITY_DN66550_c5_g1_i1 |
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| (T)10ctttttttttc(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGCAGATACTGTTTCATGGC | CGCGCAAAACTGATCAAGGT | Start: 40 |
| Tm | 59.322 | 59.762 | End: 290 |
| Size | 21 | 20 | 251 |
| Primer 2 details |
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| Sequence | GGGCAGATACTGTTTCATGGC | TCGCGCAAAACTGATCAAGG | Start: 40 |
| Tm | 59.322 | 59.483 | End: 291 |
| Size | 21 | 20 | 252 |
| Primer 3 details |
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| Sequence | TCTGATTGCACGTTTCCTCTCA | CGCGCAAAACTGATCAAGGT | Start: 125 |
| Tm | 59.963 | 59.762 | End: 290 |
| Size | 22 | 20 | 166 |
| Primer 4 details |
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| Sequence | TGCACGTTTCCTCTCATGTT | CGCGCAAAACTGATCAAGGT | Start: 131 |
| Tm | 57.745 | 59.762 | End: 290 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
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| Sequence | TTGCACGTTTCCTCTCATGT | CGCGCAAAACTGATCAAGGT | Start: 130 |
| Tm | 57.745 | 59.762 | End: 290 |
| Size | 20 | 20 | 161 |
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