ssr_primer
Primer Details for TRINITY_DN66546_c2_g3_i2 |
|---|
| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAAACAGCACAGCGCAGTG | TTACGTCCGACCTCTTTGGC | Start: 766 |
| Tm | 60.111 | 60.038 | End: 916 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | CACTAAACAGCACAGCGCAG | TTACGTCCGACCTCTTTGGC | Start: 764 |
| Tm | 60.111 | 60.038 | End: 916 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | GTCACTAAACAGCACAGCGC | TTACGTCCGACCTCTTTGGC | Start: 762 |
| Tm | 60.111 | 60.038 | End: 916 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GCCAAAGAGGTCGGACGTAA | AGCGGTTCCATTGGTTTCCT | Start: 897 |
| Tm | 60.038 | 59.888 | End: 1020 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | CTAAACAGCACAGCGCAGTG | CCCGCGGGAGAAGGTTTATT | Start: 766 |
| Tm | 60.111 | 60.107 | End: 869 |
| Size | 20 | 20 | 104 |
(C)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAAACAGCACAGCGCAGTG | TTACGTCCGACCTCTTTGGC | Start: 766 |
| Tm | 60.111 | 60.038 | End: 916 |
| Size | 20 | 20 | 151 |
| Primer 2 details |
|---|
| Sequence | CACTAAACAGCACAGCGCAG | TTACGTCCGACCTCTTTGGC | Start: 764 |
| Tm | 60.111 | 60.038 | End: 916 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | GTCACTAAACAGCACAGCGC | TTACGTCCGACCTCTTTGGC | Start: 762 |
| Tm | 60.111 | 60.038 | End: 916 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GCCAAAGAGGTCGGACGTAA | AGCGGTTCCATTGGTTTCCT | Start: 897 |
| Tm | 60.038 | 59.888 | End: 1020 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | CTAAACAGCACAGCGCAGTG | CCCGCGGGAGAAGGTTTATT | Start: 766 |
| Tm | 60.111 | 60.107 | End: 869 |
| Size | 20 | 20 | 104 |
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