ssr_primer
Primer Details for TRINITY_DN66499_c5_g2_i1 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATTCCATGACCAGCGCAAC | AGCCATCGGTATCCTTCCCT | Start: 322 |
| Tm | 59.901 | 60.105 | End: 439 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | ATTCCATGACCAGCGCAACT | AGCCATCGGTATCCTTCCCT | Start: 323 |
| Tm | 60.323 | 60.105 | End: 439 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | GATTCCATGACCAGCGCAAC | AGATACGCGGTTTCGGCTTT | Start: 322 |
| Tm | 59.901 | 60.391 | End: 459 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | GATTCCATGACCAGCGCAAC | AAGATACGCGGTTTCGGCTT | Start: 322 |
| Tm | 59.901 | 60.391 | End: 460 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | GATTCCATGACCAGCGCAAC | TAAGATACGCGGTTTCGGCT | Start: 322 |
| Tm | 59.901 | 59.543 | End: 461 |
| Size | 20 | 20 | 140 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATTCCATGACCAGCGCAAC | AGCCATCGGTATCCTTCCCT | Start: 322 |
| Tm | 59.901 | 60.105 | End: 439 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | ATTCCATGACCAGCGCAACT | AGCCATCGGTATCCTTCCCT | Start: 323 |
| Tm | 60.323 | 60.105 | End: 439 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | GATTCCATGACCAGCGCAAC | AGATACGCGGTTTCGGCTTT | Start: 322 |
| Tm | 59.901 | 60.391 | End: 459 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | GATTCCATGACCAGCGCAAC | AAGATACGCGGTTTCGGCTT | Start: 322 |
| Tm | 59.901 | 60.391 | End: 460 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | GATTCCATGACCAGCGCAAC | TAAGATACGCGGTTTCGGCT | Start: 322 |
| Tm | 59.901 | 59.543 | End: 461 |
| Size | 20 | 20 | 140 |
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